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1.
Electron. j. biotechnol ; 19(2): 20-27, Mar. 2016. ilus
Artigo em Inglês | LILACS | ID: lil-782612

RESUMO

Background: WNT4 is a protein that plays a crucial role in ovarian differentiation and development in mammals, with a relatively well understood function in mammalian gonadal differentiation. The role of WNT4 in teleost fish; however, remains unclear. In the present study, cDNAs of Wnt4a and Wnt4b were cloned and characterized in the spotted scat. The expression patterns of two Wnt4 genes in the gonads at different stages of development and in fish after treatment with 17a-methyltestosterone (MT) were investigated. Results: The tissue distribution showed that Wnt4a was expressed in various tissues, including the gonads, gills, spleen, brain, and fin. Interestingly, Wnt4b not only was expressed in the gills, brain, and spleen, but also was obviously expressed in the ovary. During gonad development, Wnt4a was highly expressed in the testis at stage I and Wnt4b was mainly expressed in the ovary at stages II-III. After MT treatment, the mRNA expression of Wnt4a increased significantly up to 40 d, and the transcript level of Wnt4b decreased at 20 d. Conclusions: These results suggest that Wnt4a may be involved in gonad development and plays a role in the process of spermatogonial proliferation. Our results also demonstrate that Wnt4b is not only expressed in the nervous system, but also in the ovary and it may be involved in ovary development of the spotted scat.


Assuntos
Animais , Proteína Wnt4/genética , Peixes/genética , Expressão Gênica , Análise de Sequência , Proteínas Wnt/genética , Reação em Cadeia da Polimerase em Tempo Real , Androgênios , Metiltestosterona
2.
The Korean Journal of Parasitology ; : 163-168, 2014.
Artigo em Inglês | WPRIM | ID: wpr-20004

RESUMO

Wnt proteins are a family of secreted glycoproteins that are evolutionarily conserved and considered to be involved in extensive developmental processes in metazoan organisms. The characterization of wnt genes may improve understanding the parasite's development. In the present study, a wnt4 gene encoding 491amino acids was amplified from cDNA of metacestodes of Taenia solium using reverse transcription PCR (RT-PCR). Bioinformatics tools were used for sequence analysis. The conserved domain of the wnt gene family was predicted. The expression profile of Wnt4 was investigated using real-time PCR. Wnt4 expression was found to be dramatically increased in scolex evaginated cysticerci when compared to invaginated cysticerci. In situ hybridization showed that wnt4 gene was distributed in the posterior end of the worm along the primary body axis in evaginated cysticerci. These findings indicated that wnt4 may take part in the process of cysticerci evagination and play a role in scolex/bladder development of cysticerci of T. solium.


Assuntos
Animais , Humanos , Sequência de Bases , Cisticercose/patologia , Cysticercus/enzimologia , DNA de Helmintos/genética , Regulação da Expressão Gênica , Hibridização In Situ , Reação em Cadeia da Polimerase em Tempo Real , Análise de Sequência de DNA , Sus scrofa , Suínos , Doenças dos Suínos , Taenia solium/embriologia , Proteína Wnt4/genética
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